Extended spectrum Beta - Lactamase (EBSL) in E.coli
isolated from a tertiary hospital in Enugu state, Nigeria

Iroha IR¹, Adikwu MU², Esimone CO³, Aibinu I⁴ Amadi ES⁵

ABSTRACT

Objective: The frequency of extended spectrum ß - lactamase (ESBL) producing E. coli in a tertiary hospital in Enugu, Enugu State, Nigeria was studied.

Methodology: Clinical specimens were collected from patients attending the Medical Microbiology laboratory unit of the University of Nigeria Teaching Hospital (UNTH), Enugu. Isolates of E. coli were obtained from various specimen types namely, urine (19), blood (25), wound (52), and sputum (32). Susceptibility studies were carried out using agar diffusion method by Kirby and Bauer, while ESBL detection on these isolates was carried out on Mueller Hinton agar using the double disc diffusion method.

Results: The frequency of ESBL producing E. coli among the clinical isolates was 11.4% (15) and ESBL producing organisms were isolated more frequently from blood (6) followed by wound (5), urine (3) and sputum (1).

Conclusions: Urgent measures to avert the further spread of ESBL producing organisms in Enugu, Enugu State, Nigeria is a compelling necessity.

KEYWORDS: ESBL, Isolate, Resistance, Sensitivity, Antibiotic discs, Frequency, Plasmid.

Pak J Med Sci    April - June 2009    Vol. 25 No. 2    279-282

How to cite this article:

Iroha IR, Adikwu MU, Esimone CO, Aibinu I Amadi ES.Extended spectrum Beta- L actamase (EBSL) in E.coli isolated from a tertiary hospital in Enugu state, Nigeria. Pak J Med Sci 2009;25(2):279-282.

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1. Iroha IR,
Department of Applied Microbiology,
Ebonyi State University
P.M.B. O53 Abakaliki, Ebonyi State, Nigeria.
2. Adikwu MU,
3. Esimone CO,
2-3: Department of Pharmaceutics,
University of Nigeria, Nsukka, Nigeria.
4. Aibinu I,
Department of Medical Microbiology and Parasitology,
College of Medicine, University of Lagos,
P.M.B. 12003, Lagos, Nigeria.
5. Amadi ES,
Department of Applied Microbiology,
Ebonyi State University P.M.B. O53,
Abakaliki, Nigeria.

Correspondence

Dr. Amadi, E.S
E-mail: amadies2001@yahoo.com

* Received for Publication: April 12, 2008
* Revision Received: January 19, 2009
* Revision Accepted: January 25, 2009

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