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Volume 23 |
April - June 2007 (Part-II) |
Number 3 |
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Published by : PROFESSIONAL MEDICAL PUBLICATIONS |
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Volume 23 |
April - June 2007 (Part-II) |
Number 3 |
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Method of quantitative bacterial
count in burn wound
Mohammad Ashraf Ganatra1, Hammad Ashraf Ganatra2
ABSTRACT
Objective: To describe a technique for conducting Quantitative bacteriology in burn wounds, which is a very important tool in the management of burn wound sepsis.
Methodology: Technique was used in 21 patients with burn wound injury. Biopsy for the bacteriology was taken with the help of punch forceps. Then it was directly put into sterilized pre weighed homogenizer bag containing 1ml normal saline. This bag was re-weighed. Weight of the tissue was obtained and bag was homogenized in homogenizer (Stomacher Lab Blender-80). Four fold dilutions were made of homogenate specimen. From each dilution tube, 0.01ml was then inoculated on blood agar plates, incubated for 24 hours and number of colonies counted by formula.
Results: Four specimens were taken each day and repeated on alternate days for up to 6 days. Out of twenty one patients there were 10 male and 11 female with mean age of 25.95 years. Total body surface area burnt was from 9-41%. Bacterial counts obtained varied from 1.45-8.89.
Conclusion: Quantitative bacteriology is simple by the above method and should be employed in every public and private sector burn center.
KEY WORDS: Burn wound, Burn sepsis, Quantitative bacteriology, Wound biopsy.
Pak J Med Sci May - June 2007 Vol. 23 No. 3 415-419
1. Mohammad Ashraf Ganatra,
Assistant professor,
Dept. of Plastic, Reconstructive & Micro Surgery,
Dow University of Health Sciences,
Dow Medical College & Civil Hospital,
Karachi.
2. Hammad Ashraf Ganatra,
Medical student, Class of 2008,
Aga Khan University Hospital,
Karachi.
Correspondence
Dr. Mohammad Ashraf Ganatra,
Suit no.9, 1st floor,
Taj Medical Complex,
M.A. Jinnah Road, Opp Capri Cinema
Karachi – Pakistan.
E-mail: ganatra@cyber.net.pk
* Received for Publication: October 31, 2006
* Revision Received: February 9, 2007
* Revision Accepted: February 28, 2007
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