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Volume 25 |
April - June 2009 (Part-II) |
Number 3 |
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Published by : PROFESSIONAL MEDICAL PUBLICATIONS |
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ISSN 1681-715X |
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ORIGINAL ARTICLE |
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Volume 25 |
April - June 2009 (Part-II) |
Number 3 |
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Prevalence of EHEC 0157:H7 from human and
environmental samples from Lagos and Zaria
Smith SI1, Bello OS2, Goodluck HA3,
Omonigbehin EA4, Agbogu VN5, Odeigah P6
ABSTRACT
Objective: Prevalence, antibiotic patterns and plasmid profiles of Enterohaemorrhagic Escherichia coli (EHEC) 0157:H7 from humans and environmental sources were studied at various seasons of the year in Nigeria.
Methodology: Stool and environmental samples were obtained from Lagos and Zaria and characterized as EHEC 0157:H7 using the method of Cowan and serotyping. The isolates were further characterized using antibiotic susceptibility testing and plasmid profile.
Results: Out of 132 stool samples, 42 (31.8%) of EHEC 0157:H7 were isolated between April to December 2004. These samples were from Lagos (Western Nigeria) and Zaria (Northern Nigeria). Thirty-three isolates were from Lagos while 9 were from Zaria. Out of 90 environmental samples, 11 (12.2%) were positive for EHEC 0157:H7. The highest number of isolates (13) was obtained during the month of December, the dry, harmattan and festive season. Most of the isolates from Zaria were resistant to ampicillin, nitrofurantoin and tetracycline, while those from Lagos were resistant to ampicillin, cotrimoxazole, nitrofurantoin and tetracycline. Nine out of 53 (17%) isolates harboured detectable plasmids.
Conclusion: The presence of multi-drug resistance amongst these isolates is of concern and so antibiotic therapy should not be recommended as it complicates the disease while misuse in our environment has led to emergence of resistance strains.
KEY WORDS: EHEC 0157:H7, Plasmids, Lagos, Zaria, Antibiotic susceptibility patterns.
Pak J Med Sci April - June 2009 (Part-II) Vol. 25 No. 3 398-403
How to cite this article:
Smith SI, Bello OS, Goodluck HA, Omonigbehin EA, Agbogu VN, Odeigah P. Prevalence of EHEC 0157:H7 from human and environmental samples from Lagos and Zaria. Pak J Med Sci 2009;25(3): 398-403.
1. Smith SI,
2. Bello OS,
3. Goodluck HA,
4. Omonigbehin EA,
1,3,4: Molecular Biology and Biotechnology Division,
Nigerian Institute of Medical Research,
6-Edmond Crescent, Yaba, Lagos, Nigeria.
5. Agbogu VN,
Department of Microbiology,
Ahmadu Bello University, Zaria, Nigeria.
6. Odeigah P
2,6: Department of Cell Biology and Genetics,
University of Lagos, Nigeria.
Correspondence
Dr. SI Smith,
Email: stellaismith@yahoo.com
* Received for Publication: October 7, 2008
* Revision Received: April 23, 2009
* Revision Accepted: May 4, 2009
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