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Published by : PROFESSIONAL MEDICAL PUBLICATIONS |
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ISSN 1681-715X |
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ORIGINAL ARTICLE |
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Volume 25 |
April - June 2009 (Part-II) |
Number 3 |
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Effect of Fetal Calf Serum on Cellular Proliferation of
Mouse Y1 Adrenocortical Cells in vitro
Muhammad Imran Naseer1, Hassan Zubair2, Ikramullah3, Myeong ok Kim4
ABSTRACT
Objective: Fetal calf serum (FCS) is a supplement used in cell culture media for successful culturing. The present study was design to observe the effect of FCS on cellular proliferation.
Methodology: Effect of different concentrations (0, 1, 5, 10 and 20%v/v) of FCS on cellular proliferation was determined by the uptake of crystal violet, MTT assay. Total cellular protein was also measured colorimetrically to observe the effect of FCS on growth of mouse Y1 adrenocortical cells.
Results: The results showed a gradual increase in proliferation of Y1 cells by increasing concentrations of FCS in Dulbecco’s modification of Eagle’s medium (DMEM). Highest proliferation rate of Y1 adrenocortical cells was achieved with cultured cells after 6 days in DMEM medium containing 10 to 20% FCS.
Conclusion: The study suggested that supplementation of 20% FCS increase cell proliferation and acts as a growth factor results in cell division and DNA synthesis.
KEYWORDS: FCS, cell proliferation. Y1 adrenocortical cells.
Pak J Med Sci April - June 2009 (Part-II) Vol. 25 No. 3 500-504
How to cite this article:
Naseer MI, Zubair H, Ikramullah, Kim MO. Effect of Fetal Calf Serum on Cellular Proliferation of Mouse Y1 Adrenocortical Cells in vitro. Pak J Med Sci 2009;25(3): 500-504.
1. Muhammad Imran Naseer, PhD
Gyeongsang National University,
Division of Life Science (BK 21),
Chinju 660-701, South Korea
2. Hassan Zubair, MS
Sheffield Hallam University,
Sheffield, S1 1WB, United Kingdom.
* Muhammad Imran Naseer & Hassan Zubair contributed equally to this work.
3. Ikramullah, PhD
Gyeongsang National University,
Division of Life Science (BK 21),
Chinju 660-701, South Korea.
4. Prof. Myeong Ok Kim, Ph.D
Division of Life Science, College of Natural Science
Gyeongsang National University 900,
Gazwa 351-301 Chinju 660-701, South Korea
Correspondence:
Prof. Myeong Ok Kim, Ph.D
E-mail: mokim@gsnu.ac.kr
* Received for Publication: December 31, 2008
* Revision Received: June 4, 2009
* Revision Accepted: June 5, 2009
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