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Published by : PROFESSIONAL MEDICAL PUBLICATIONS |
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ISSN 1681-715X |
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ORIGINAL ARTICLE |
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Volume 23 |
July - September 2007 |
Number 4 |
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Analysis of human gallstones by
Fourier Transform Infrared (FTIR)Naseem A. Channa1, Fateh D. Khand2, Tayab U Khand3,
Mhhammad H. Leghari4, Allah N Memon5ABSTRACT
Objective: The present study was aimed at determining the composition of gallstones removed from patients in Southern Sindh, Pakistan.
Methodology: One hundred nine (109) gallstone samples surgically removed from as many patients (98 females and 11 males; age range 20 to 80 years) admitted for treatment in Liaquat University hospital, Jamshsoro during 2000 to 2003, were analyzed for composition by Fourier Transform Infrared (FTIR) spectroscopy.
Results: Seventy four (67.9%) of the 109 gallstone samples were found to be pure cholesterol stones, Five (4.6%) pure calcium carbonate stones, Thirteen (11.9%) cholesterol + calcium carbonate, Ten (9.2%) cholesterol + bilirubin and Seven (6.4%) calcium bilirubinate stones. In mixed composition gallstones cholesterol was concentrated more at periphery than in the center of stone. Cholesterol either singly (67.9%) or in combination with either calcium carbonate (11.9%) or bilirubin (9.2%) was the most predominant component of gallstones.
Conclusion: Analysis of gallstones based on FTIR suggests that cholesterol either singly or in combination with either calcium carbonate or bilirubin is the most predominant component of gallstones in Southern Sindh, Pakistan.
KEY WORDS: Gallstones, Composition, FTIR, Cholesterol, Bilirubin, Calcium bilirubinate, Calcium carbonate, mixed gallstones.
Pak J Med Sci July - September 2007 Vol. 23 No. 4 546-550
1. Naseem A. Channa, Ph.D.
2. Fateh D. Khand, Ph.D.
Department of Biochemistry,
Isra University, Hyderabad
3. Tayab U Khand, MBBS
Department of Biochemistry,
Liaquat University of Medical & Health Sciences,
Jamshoro - Pakistan.
4. Mhhammad H. Leghari, FRCS
1,4: Department of Surgery,
Isra University, Hyderabad
5. Allah N Memon, Ph.D.
1-5: Institute of Biochemistry, University of Sindh,
Jamshoro – 71000,
Pakistan.
Correspondence
Dr. Naseem Aslam Channa
Assistant Professor, Institute of Biochemistry
University of Sindh, Jamshoro – 71000,
Hyderabad – Pakistan.
Email address: nachanna2000@yahoo.com
* Received for Publication: January 4, 2007
* Revision Received: April 26, 2007
* Revision Accepted: May 2, 2007
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