|
|
||||
|
Published by : PROFESSIONAL MEDICAL PUBLICATIONS |
||||
|
ISSN 1681-715X |
||||
|
||||
|
|
||||
|
- |
||||
|
ORIGINAL ARTICLE |
||||
|
- |
||||
|
Volume 25 |
July - September 2009 |
Number 4 |
||
|
|
||||
|
||||
rK39 enzyme-linked immunosorbent assay for the
diagnosis of Kala-azar in an endemic zone of Bangladesh
M. Abdus Salam1, Dinesh Mondal2, Mamun Kabir3, Rashidul Haque4
Abstract
Objective: The diagnostic role of rK39 enzyme linked immunosorbent assay (ELISA) was compared with the buffy-coat Leishmania nested polymerase chain reaction (Ln-PCR) for kala-azar in an endemic zone of Bangladesh.
Methodology: This evaluation study included 100 cases comprising of 60 clinically suspected patients of kala-azar and 40 healthy controls. Anti-rK39 antibody was estimated in plasma by ELISA and buffy coat was utilized to detect Leishmania DNA by Ln-PCR from both patients and controls. The diagnostic role of rK39 ELISA was then compared with Ln-PCR positive cases which were considered as confirmed cases of kala-azar.
Results: Out of 60 suspected patients of kala-azar, 58 (96.67%) were found positive in ELISA against 56 (93.33%) of Ln-PCR-positive cases. Further, 55 (98.21%) of ELISA-positive patients were also found positive in Ln-PCR. Voluntary healthy controls (40) included 20 persons from the endemic zone and 20 from non-endemic zone of kala-azar, were all found negative in both Ln-PCR and rK39-ELISA. The sensitivity and specificity of ELISA were found to be 98.21% and 95.65% respectively.
Conclusion: rK39-ELISA is a non-invasive serodiagnostic tool for visceral leishmaniasis with very high sensitivity and specificity.
KEY WORDS:
Visceral leishmaniasis, Kala-azar, rK39-ELISA, Ln-PCR, Sensitivity, Specificity.Pak J Med Sci July - September 2009 Vol. 25 No. 4 635-640
How to cite this article:
Salam MA, Mondal D, Kabir M, Haque R. rK39 enzyme-linked immunosorbent assay for the diagnosis of Kala-azar in an endemic zone of Bangladesh. Pak J Med Sci 2009;25(4):635-640.
1. Dr. Md. Abdus Salam, M.Phil (Microbiol.),
M.Sc (UK)
Assistant Prof., Dept. of Microbiology,
Rajshahi Medical College, Rajshahi 6000, Bangladesh.
2. Dr. Dinesh Mondal, PhD
Assistant Scientist, Parasitology Laboratory,
ICDDR,B, Dhaka, Bangladesh.
3. Mamun Kabir, M.Sc
Research Officer, ICDDR, B
4. Dr. Rashidul Haque, PhD
Head, Parasitology Laboratory, ICDDR, B,
Dhaka, Bangladesh.
Correspondence
Dr. Md. Abdus Salam, M.Phil (Microbiol.), M.Sc (UK)
Assistant Prof. of Microbiology, Rajshahi Medical College,
Rajshahi-6000 – Bangladesh.
E-mail: drsalamrmc@yahoo.com
* Received for Publication: November 1, 2008
* Accepted: June 25, 2009