Pakistan Journal of Medical Sciences

Published by : PROFESSIONAL MEDICAL PUBLICATIONS

ISSN 1681-715X

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ORIGINAL ARTICLE

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Volume 22

October - December 2006

Number 4


 

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In vitro Synergism Between Miconazole and
Griseofulvin Against Candida Species

Ali Zarei Mahmoudabadi1, Farshad Farrahei2, Majid Zarrin3

Abstract

Objective: To find the synergistic effect between miconazole and griseofulvin on common human Candida.

Methods: A serial dilution of miconazole and griseofulvin in Yeast Nitrogen Base (YNB) was prepared in test tubes. 50μl of standardised suspension of yeasts was inoculated into each test tube and incubated at 30oC for 24-48 hours. The antifungal activity of miconazole alone or with griseofulvin was measured in vitro against 300 isolates of the Candida.

Results: Miconazole and griseofulvin in combination showed a Minimum Inhibitory Concentration (MIC) of 6.7mg/l miconazole (M) and 20mg/l griseofulvin (G) for C. albicans, 13mg/l M and 20mg/l G for C. tropicalis, 4.9mg/l M and 20mg/l G for C. pseudotropicalis and 14.2mg/l M and 20mg/l G for C. parapsilosis. The combination of miconazole with griseofulvin resulted in synergistic activity (Fractional Inhibitory Concentration, FIC <0.82) against C. albicans in a microdilution checkerboard assay. FICs for other species were as follow: FIC <0.75 for C. tropicalis, FIC <0.86 for C. pseudotropicalis and FIC <0.79 for C. parapsilosis.

Conclusion: These results suggest that combination of griseofulvin and miconazole regimens may reduce usual dosage of miconazole and it is helpful in the control of infections caused by Candida species.

Key Words: Candida, Miconazole, Griseofulvin, Synergism.

Pak J Med Sci October - December 2006 Vol. 22 No. 4 454-456


1. Dr. Ali Zarei Mahmoudabadi

2. Dr. Farshad Farrahei

3. Dr. Majid Zarrin

1-3. Department of Medical Mycology,
Jundishapour University of Medical Sciences,
Ahwaz,
Iran.

Correspondence:
Dr. Ali Zarei Mahmoudabadi
Email: zarei40@hotmail.com

* Received for Publication: September 29, 2005

* Accepted: April 30, 2006


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