Pakistan Journal of Medical Sciences

Published by : PROFESSIONAL MEDICAL PUBLICATIONS

ISSN 1681-715X

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ORIGINAL ARTICLE

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Volume 23

October - December 2007 (Part-II)

Number  6


 

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Role of enriched media in bacterial isolation from semen and effect of
microbial infection on semen quality: A study on 100 infertile men

Orji Ikechukwu1, Ezeifeka George2, Amadi Ekperechi Sabinus3, Okafor Florence4

ABSTRACT

Objective: The study was undertaken to determine the isolation potential of solid media with and without pre-enrichment as well as the possible influence of microbial infection on semen quality.

Methodology: Semen samples of 100 infertile men were cultured on solid media with and without complementation with liquid media culture (pre-enrichment) respectively. The cultured plates were examined macroscopically and the colonies identified. The sperm count, motility and morphological characteristics of the semen samples were also determined.

Results: The bacteria recovered from both cultural methods includes Staphylococcus aureus, Staphylococcus epidermidis, ß-haemolytic streptococci, α-haemolytic streptococci, Escherichia coli, Proteus and Klebsiella species. There was a significant increase in bacterial isolation when solid media culture was pre-enriched than when the former was used alone (p<0.05). There was no significant  relationship between bacteria isolation and ranges of total sperm count, sperm motility, morphological abnormalities and age groups studied (p<0.05).

Conclusion: Pre-enriched solid media is a more efficient technique for optimal bacterial isolation from seminal fluid.

KEYWORDS: Semen, Pre-enrichment, Culture, Abakaliki.

Pak J Med Sci    October - December 2007 (Part-II)    Vol. 23 No. 6    885-888


1. Mr. Orji Ikechukwu
Dept. of Medical Microbiology, Ebonyi State University
Teaching Hospital Abakaliki, Nigeria.
2. Prof. Ezeifeka George,
Department of Applied Microbiology and Brewing,
Nnamdi Azikiwe University, Awka – Nigeria.
3. Dr. Amadi Ekperechi Sabinus,
Dept. of Applied Microbiology, Faculty of Applied and
Natural Sciences,Ebonyi State University,
P.M.B, 53 Abakaliki, Ebonyi State, Abakaliki – Nigeria.
4. Mrs. Okafor Florence,
Dept. of Medical Microbiology, Ebonyi State University
Teaching Hospital, Abakaliki – Nigeria.

Correspondence

Dr. Amadi Ekperechi Sabinus,
E-mail: amadies2001@yahoo.com

* Received for Publication: March 24, 2007
* Revision Received: May 14, 2007
* Revision Accepted: September 27, 2007



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