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Published by : PROFESSIONAL MEDICAL PUBLICATIONS |
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ISSN 1681-715X |
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ORIGINAL ARTICLE |
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Volume 25 |
October - December 2009 (Part-II) |
Number 6 |
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Effects of adenosine on the organ injury and
dysfunction caused By Hemorrhagic Shock
Mona M. Soliman1
ABSTRACT
Objectives: Adenosine has been shown in animal and human studies to decrease the post-ischemic myocardial injury by lowering the levels of tumor necrosis factor-a. The objectives of the study was to examine the protective effects of adenosine on the organ injury (liver, kidney, pancreas) associated with hemorrhagic shock in rats.
Methodology: The study was conducted at Cardiovascular Physiology laboratory, King Saud University, Riyadh in 2007-2008. Anesthetized male Sprague- Dawley rats were assigned to hemorrhage and resuscitation treated with 20mM adenosine , untreated, or similar time matched control groups (n=6 per group). Rats were hemorrhaged for one hour using a reservoir model. Arterial blood pressure was monitored for one hour, and maintained at a mean arterial blood pressure of 40 mmHg. Adenosine 20mM was injected intra-arterially, before resuscitation in the adenosine treated group. Resuscitation was performed by reinfusion of the sheded blood for 30 minutes. Arterial blood samples were analyzed for biochemical indicators of multiple organ injury: 1) liver function: aspartate aminotransferase (AST), alanine aminotransferase (ALT), 2) renal function: urea and creatinine, 3) pancreatic function: amylase.
Results: In the control group there was no significant rise in the serum levels of (i) urea and creatinine, (ii) aspartate aminotransferase (AST) and alanine aminotransferase (ALT), (iii) amylase. While in the adenosine treated group, resuscitation from one hour of hemorrhagic shock resulted in significant rises in the serum levels of (i) urea and creatinine , (ii) aspartate aminotransferase (AST) and alanine aminotransferase (ALT), (iii) amylase. Treatment of rats with 20mM adenosine before resuscitation following one hour of hemorrhagic shock decreased the multiple organ injury and dysfunction caused by hemorrhagic shock.
Conclusion: Adenosine attenuated the renal, liver and pancreatic injury caused by hemorrhagic shock and resuscitation in rats. Thus, the inflammatory response to shock may contribute to the multiple organ failure developed after hemorrhagic shock and resuscitation.
KEY WORDS:
Adenosine, hemorrhagic shock, multiple organ failure, TNF-a, inflammation.Pak J Med Sci October - December 2009 (Part-II) Vol. 25 No. 6 890-894
How to cite this article:
Soliman MM. Effects of adenosine on the organ injury and dysfunction caused By Hemorrhagic Shock. Pak J Med Sci 2009;25(6):890-894.
1. Dr. Mona M. Soliman, MBBS, MSc., PhD
Assistant Professor, Dept. of Physiology (29)
College of Medicine, King Khalid University Hospital,
Riyadh 11461, Saudi Arabia.
Correspondence:
Dr. Mona M. Soliman, MBBS, MSc., PhD
Email: monaslmn@yahoo.com
* Received for Publication: May 21, 2009
* Revision Received: October 12, 2009
* Accepted: October 21, 2009
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