Molecular epidemiology and phylogenetic analysis of Dengue virus type-1 and 2 isolated in Malaysia
Objective: Detection of different serotypes of dengue virus and provide information on origin, distribution and genotype of the virus.
Methods: Dengue virus serotypes identified as DEN-1 and DEN-2 were amplified and sequenced with E gene. The consensus sequences were aligned with references E gene sequences of globally available GenBank. Phylogenetic analysis was performed using Neighbor-joining and Kimura 2-parameter model to construct phylogenetic tree.
Results: A total of 53 dengue virus isolates were positive, of which 38 (71.7%) were DENV-1 and 15 (28.3%) were DENV-2. Phylogenetic tree of DENV-1 and DENV-2 showed that the isolates were clustered in genotype I and cosmopolitan genotype, respectively considered the predominant genotypes in Southeast Asian countries. The molecular epidemiology genotype I DENV-1 and cosmopolitan genotype DENV-2 have been co-circulating in Klang Valley areas, Malaysia without shifting of genotype.
Conclusion: The study reveals that DENV-1 and DENV-2 have been circulating in Malaysia. The isolates are clustered in genotype 1 and cosmopolitian genotype, respectively. The study results would help in planning for prevention and control of dengue virus in Malaysia.
How to cite this:Chew MH, Rahman MM, Hussin S. Molecular epidemiology and phylogenetic analysis of Dengue virus type-1 and 2 isolated in Malaysia. Pak J Med Sci 2015;31(3):615-620. doi: http://dx.doi.org/10.12669/pjms.313.6340
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