Imipenem Resistant Pseudomonas aeruginosa:The fall of the final quarterback | Ameen | Pakistan Journal of Medical Sciences Old Website

Imipenem Resistant Pseudomonas aeruginosa:The fall of the final quarterback

Nadya Ameen, Zahida Memon, Shehla Shaheen, Ghulam Fatima, Farah Ahmed


Objective: To isolate, determine the frequency, and study the demographic trends of MBL positive Pseudomonas aeruginosa from imipenem resistant isolates collected from clinical samples in a tertiary care hospital of Pakistan.

Methods: In this cross sectional study a total of 230 strains of Pseudomonas were isolated from various clinical specimens on the basis of culture and biochemical tests. Imipenem resistant isolates were selected by Kirby Bauer Diffusion technique, followed by screening for MBL production by Imipenem EDTA Combined Disk Test. Demographic details of each patient were recorded on a separate questionnaire. Chi-Square goodness-of-fit test was computed to review the isolation of MBL positive isolates (P-value ≤ 0.05) in different specimen.

Results: Out of 230 strains of P. aeruginosa 49.5% were imipenem resistant; MBL production was confirmed in 64.9% of the resistant isolates. Resistance to polymyxin B (12.5%) was notable. Majority of the MBL positive strains were isolated from patients aged between 20-39 years (45.9%) and the predominant source was pus (43.24%) which was found to be statistically significant (P-value=0.04). Outpatient departments (24.3%) and burn unit (21.6%) were the major places for resistant isolates.

Conclusion: MBL production is one of the major causes of IRPA. Increasing resistance to polymyxin B is grave. Due to acquisition of MBL strains MDR P. aeruginosa has become endemic in tertiary setups.


How to cite this:Ameen N, Memon Z, Shaheen S, Fatima G, Ahmed F. Imipenem Resistant Pseudomonas aeruginosa:The fall of the final quarterback. Pak J Med Sci 2015;31(3):561-565.   doi:

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Full Text: PDF


  • There are currently no refbacks.