Clinical significance of quantitative and qualitative detection of BK and JC virus in blood and urine of renal transplantation recipients
Objective: To evaluate value of quantitative and qualitative detection of BK virus (BKV) and JC virus (JCV) in timely diagnosing polyomavirus-associated nephropathy (PVAN) occurring inrenal transplantation recipients.
Methods: We collected 306 cases of urine specimen and 310 cases of blood specimen from 306 patients who underwent renal transplant. Levels of BKV and JCV in blood and urine were detected using real-time quantitative polymerase chain reaction (PCR).
Results: Detection rate of BKV DNA was 33.3% (102/306) in urine and 34.8% (108/310); while that of JCV DNA was 30.7% (94/306) and 33.5% (104/310) respectively. The lowest detectable limit of BCK and JCV detection for patients who underwent renal transplant was 2×103 copies/ml, suggesting high specificity and sensitivity.
Conclusion: Real-time quantitative PCR is able to monitor BCV and JCV in renal transplant recipients in a convenient and rapid way, thus it is beneficial for early discovery, diagnosis and treatment of PVAN.
How to cite this:Qiao L, Qu Q, Jiang X. Clinical significance of quantitative and qualitative detection of BK and JC virus in blood and urine of renal transplantation recipients. Pak J Med Sci. 2016;32(2):435-439. doi: http://dx.doi.org/10.12669/pjms.322.8978
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- There are currently no refbacks.