Measured versus calculated LDL-cholesterol in subjects with Type 2 Diabetes | Fawwad | Pakistan Journal of Medical Sciences Old Website

Measured versus calculated LDL-cholesterol in subjects with Type 2 Diabetes

Asher Fawwad, Rubina Sabir, Musarrat Riaz, Hassan Moin, Abdul Basit


Objective: There is a strong positive association between increased low-density lipoprotein cholesterol (LDL-C) and coronary heart disease (CHD). The accuracy of LDL-C estimation is essential and critically important. The aim of present study was to compare calculated LDL-C with direct homogeneous assay in patients with type 2 diabetes.

Methods: This observational study was carried out at Baqai Institute of Diabetology and Endocrinology (BIDE) from January 2011 to December 2013. A total of 9620 patients with type 2 diabetes were included in the study. Fasting blood glucose, total Cholesterol, triglyceride, HDL cholesterol and LDL cholesterol were obtained using standard methods. Calculated LDL-C was obtained by Friedewald formula.

Results: Mean difference of measured and calculated LDL-C was found to be -0.25, 6.63 and 46.55 mg/dl at triglyceride levels < 150 mg/dl, 150 - 400 mg/dl and ≥ 400 mg/dl, respectively. The result shows that the difference between measured and calculated LDL-C increases as the triglyceride level increases.

Conclusions: The findings of our study suggested that calculated LDL-C was lower, as compared to measured LDL-C, which may cause misclassifications that may have an impact on therapeutic decisions in patients with diabetes. Calculated LDL-C may depend on triglyceride levels so LDL-C should be measured by direct assay in routine clinical laboratories.


How to cite this:Fawwad A, Sabir R, Riaz M, Moin H, Basit A. Measured versus calculated LDL-cholesterol in subjects with Type 2 Diabetes. Pak J Med Sci. 2016;32(4):955-960.   doi:

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Full Text: PDF


  • There are currently no refbacks.