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Volume 26 |
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Number 1 |
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Volume 26 |
January - March 2010 |
Number 1 |
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Molecular characterization of VP7, VP4, and NSP4 genes of P[8]G9
rotavirus strains detected in Saudi Arabia: First characterization
of Phylogenetic and Sequencing analysis in the Middle EastAli M Kheyami1
ABSTRACT
Objectives: Serotype G9 rotaviruses have emerged as one of the leading causes of gastroenteritis in children worldwide since the mid-1990s, suggesting that G9 is a globally important serotype.
Methodology: The molecular epidemiology of G9 rotaviruses in two Saudi’s cities, Maddina and Gizan, from 2004 to 2005, was investigated in this study.
Results: These G9 strains belonged to P[8] lineage 2 (P-like) VP4, genetic group B (Wa-like) NSP4, and long electropherotype. The Saudi Arabian G9 VP7 gene had a 98% nucleotide sequence identity and grouped with the recently circulated G9 strains belonged to the third lineage.
Conclusion: Molecular characterization of VP7, VP4, NSP4 and PAGE suggest that rotavirus G9 could have been introduced to Saudi as a novel G9P[8] strain. This study is the first to describe Molecular characterization of G9 Strains in the Middle East.
KEY WORDS:
Rotavirus, G9, Saudi Arabia.Pak J Med Sci January - March 2010 Vol. 26 No. 1 31-35
How to cite this article:
Kheyami AM. Molecular characterization of VP7, VP4, and NSP4 genes of P[8]G9 rotavirus strains detected in Saudi Arabia: First Characterization of Phylogenetic and Sequencing Analysis in the Middle East. Pak J Med Sci 2010;26(1):31-35
1. Dr. Ali M Kheyami, B.Sc, M.Sc, PhD
Main Lab,
King Fahd Hospital,
Maddina,
Saudi Arabia.
Correspondence:
Dr. Ali M Kheyami,
B.Sc, M.Sc, PhD
P.O. Box: 2642,
Madina, Saudi Arabia.
E-mail: kheyami@yahoo.com
* Received for Publication: June 15, 2009
* Revision Received: June 20, 2009
* 2nd Revision Received: November 24, 2009
* Final Revision Accepted: November 26, 2009
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