Pakistan Journal of Medical Sciences

Published by : PROFESSIONAL MEDICAL PUBLICATIONS

ISSN 1681-715X

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ORIGINAL ARTICLE

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Volume 22

October - December 2006

Number 4


 

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Comparison of Different laboratory Methods for Detection
of Methicillin Resistant
Staphylococcus Aureus

Mohammad Rahbar1, Mahmood Yaghoobi2, Ali Fattahi3

ABSTRACT

Objective: The aim of this study was to compare four different laboratory methods for detection of methicillin resistant Staphylococcus aureus (MRSA).

Design: The clinical specimens including urine, blood, wound and tracheal tube aspirates were processed for isolation of S .aureus.

Setting: The samples were obtained from patients admitted in Milad hospital in Tehran,Iran.

Subjects: 95 strains of S.aureus were tested with four different methods i.e. disk diffusion, oxacillin screen agar E-test and latex agglutination for methicillin resistantance.

Results: Of 95 tested S.aureus; E-test revealed that 51 isolates were MRSA. Oxacillin screen agar showed two false positive MRSA. The sensitivity and specificity of oxacillin screen agar method was 96% and 95% respectively. The MRSA –Screen latex agglutination showed 54(three false positive) MRSA. The sensitivity and specificity for this method was 94% and 93% respectively. Results of susceptibility testing by disk diffusion methods in comparison with other methods were conflicting.

Conclusions: Oxacillin screen agar test is a reliable alternative for detection of MRSA in clinical laboratory where MIC detection or molecular methods are not available.Also MRSA latex agglutination kit offers an interesting new approach to early detection. of MRSA.

KEY WORDS: Staphyloccus aureus, MRSA, Oxacillin.

Pak J Med Sci October - December 2006 Vol. 22 No. 4 442-445


1. Dr. Mahammad Rahbar
Department of Microbiology,
Bo Ali Hospital, Damavand Avenue,
P.O. Box: 17115-365,
Tehran – Iran.

2. Dr. Mahmood Yaghoobi,

3. Ali Fattahi,

2-3: Department of Microbiology,
Hemmat Highway, Milad Hospital,
Department of Infection Control Committee,
P.O. Box: 1455-4189,
Tehran – Iran.

Correspondence:
Dr. Mohammad Rahbar,
E-Mail: mhhf_rz@yahoo.com

* Received for Publication: September 24, 2005

* Revision Received: May 15, 2006

* Accepted: June 20, 2006


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