Pakistan Journal of Medical Sciences

Published by : PROFESSIONAL MEDICAL PUBLICATIONS

ISSN 1681-715X

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ORIGINAL ARTICLE

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Volume 26

October - December 2010

Number  4


 

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PER-1-type extended-spectrum -lactamase producing
Acinetobacter baumannii isolated from
clinical specimens in Tabriz, Iran

Nasrollah Sohrabi1, Mohammad Taghi Akhi2, Safar Farajnia3,
Mohammad Reza Nahaei4, Mohammad Ahangarzadeh Rezaei5

ABSTRACT

Objective: To determine the prevalence of PER-1 gene type ESBLs producing isolates of A. baumannii in clinical specimens.

Methodology: During March 2008 to June 2009, a total of 100 A. baumannii isolates were recovered from clinical specimens of hospitalized patients in Imam Reza hospital in Tabriz. These isolates were subjected to susceptibility tests using five selected cephalosporins according to CLSI guidelines. Screening for ESBL production was carried out by confirmatory tests including DDST and CDM. The prevalence of PER-1 gene in these isolates was also determined by using PCR.

Results: All of A. baumannii isolates in this study were resistant at least to one of the selected cephalosporins. Sixty four percent of isolates exhibited a >5 mm zone size enhancement in the CDM test, whereas 53% of them gave positive results by DDST as ESBL producer. Collectively CD method and DDST showed 70% of A. baumannii as ESBLs producer, of which 51% had PER-1 gene.

Conclusion: This research shows high prevalence of PER-1 gene type ESBLs producing A. baumannii isolates in Tabriz which is strong evidence for their resistance to cephalosporins.

KEY WORDS: A. baumannii, ESBL, PCR, DDST, CDM.

Pak J Med Sci    October - December 2010    Vol. 26 No. 4    769-772

How to cite this article:

Sohrabi N, Akhi MT, Farajnia S, Nahaei MR, Rezaei MA. PER-1-type extended-spectrum -lactamase producing Acinetobacter baumannii isolated from clinical specimens in Tabriz, Iran. Pak J Med Sci 2010;26(4): 769-772


1. Nasrollah Sohrabi,
PhD Student of Medical Bacteriology.
2. Mohammad Taghi Akhi,
PhD of Medical Microbiology, Associate Professor.
3. Safar Farajnia,
PhD of Biotechnology, Assistant Professor.
4. Mohammad Reza Nahaei,
PhD of Medical Microbiology, Professor of Microbiology.
5. Mohammad Ahangarzadeh Rezaei,
PhD of Medical Microbiology, Assistant Professor.
1,3: Drug Applied Research Center & Biotechnology Research Center.
2,4,5: Department of Microbiology, Faculty of Medicine.
1-5: Tabriz University of Medical Sciences, Tabriz, Iran.

Correspondence:
Mohammad Taghi Akhi,
PhD of Medical Microbiology,Dept. of Microbiology,
Faculty of Medicine and Drug Applied Research Center,
Tabriz University of Medical Sciences, Iran.
E-mail: M_T_Akhi@yahoo.com, Akhim@tbzmed.ac.ir

* Received for Publication: February 8, 2010
* Revision Received: July 1, 2010
* Revision Accepted: July 5, 2010
 



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